8C9

99072805, Reactivity: Anti-CD36, human

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen,female
• 골수종: NS-1
• 반응성: Anti-CD36, human
• 기술: cell culture | mammalian: suitable
• 동형: IgM
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse x Mouse hybridoma

세포주 설명

The hybridoma line 8C9 was produced by immunizing a female BALB/c mouse i.p. with tissue from a human malignant fibrous histiocytoma once a week for 6 weeks. Mouse spleen cells were fused with myeloma NS-1 cells using PEG and HAT for selection . The 8C9 monoclonal antibody detects a unique epitope on the leukocyte differentiation antigen CD36 (platelet glycoprotein IV or III6) expressed by normal and neoplastic endothelial cells. Immunoreactivity with 8C9 was detected on capillary endothelial cells, adipocytes, monocytes, platelets and the human monocytoid cell line U-937 (Sigma Catalogue number. 85011440). The antibody did not react with erythroid cells, T cells, B cells or HUVECs (human umbilical vein endothelial cells). Immunoblot analysis showed that 8C9 bound to a 97-kDa membrane protein expressed by U-937 cells treated with phorbol ester. Comparative immunohistochemical studies using 8C9 and the monoclonal antibody OKM demonstrated epitopic heterogeneity of the CD36 antigen by normal and neoplastic endothelial cells.

면역원

Human malignant fibrous histiocytoma

애플리케이션

Study of endothelial cells, CD36 expression analysis

배양 배지

RPMI 1640 + 2mM glutamine + 10% FBS

계대배양 정규 작업

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.