BHL-89

98070109, human lymph (axillary), Lymphoblastoid

• UNSPSC 코드: 41106514

속성

• product name: BHL-89, 98070109
• 생물학적 소스: human lymph (axillary)
• 성장 모드: Suspension
• 핵형: Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+
• 형태학: Lymphoblastoid
• 제품: Not specified
• 수용체: sIgM,CD22,BB1,HLA-ABC,GnRH
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human B-type EBNA negative lymphoma

세포주 설명

The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.

애플리케이션

B lymphoma studies, cancer treatments

배양 배지

RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Maintain cultures between 1-5 x 100,000 cells/ml;5% CO₂; 37°C

기타 정보

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