BC9

95030301, Reactivity: Anti-TNF, bovine

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: Mouse x Mouse Hybridoma
• 포장: tube of 5 μg 95030301-DNA-5UG; pkg of vial of cells 95030301-1VL
• ImmunoDon: BALB/c mouse spleen
• 골수종: P3X63Ag8.653
• 반응성: Anti-TNF, bovine
• 기술: cell culture | mammalian: suitable
• 동형: IgG1
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse P3X63Ag8.653 x Mouse BALB/c spleen

세포주 설명

The monoclonal antibody BC9 detects bovine tumour necrosis factor (TNF) in a radioimmunoassay with a detection limit of 24 pg/ml. Bovine recombinant TNF can be neutralised in vitro by addition of BC9. Cytotoxic assays showed a neutralising effect using L929 and WEHI 164 clone 13 cells.

면역원

Bovine recombinant TNF-alpha

애플리케이션

Detection of TNF in bioassay or radioimmunoassay, in vivo studies (neutralisation of TNF).

배양 배지

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

계대배양 정규 작업

Maintain cultures between 5-6x100,000 cells/ml; 5% CO₂; 37°C.

기타 정보

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