VA-ES-BJ

94092303, human skin (bone marrow metastasis), Epithelial

• UNSPSC 코드: 41106514

속성

• product name: VA-ES-BJ, 94092303
• 생물학적 소스: human skin (bone marrow metastasis)
• 성장 모드: Adherent
• 핵형: 66-68 Aneuploidic, pentasomy #7, multiple trisomy
• 형태학: Epithelial
• 제품: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
• 수용체: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): metastasis
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human skin epithelioid sarcoma

세포주 설명

Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

애플리케이션

Study of tumours. Tumourigenic in nude mice.

배양 배지

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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