RC-4B/C1

94020810, rat brain, Epithelial-like

• UNSPSC 코드: 41106514

속성

• product name: RC-4B/C1, 94020810
• 생물학적 소스: rat brain
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial-like
• 제품: Gonadatrophs
• 수용체: Gonadotrophin-releasing hormone receptors
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat pituitary adenoma

세포주 설명

RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSß, LHß and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.

애플리케이션

Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.

배양 배지

DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:6 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 10% CO₂; 34°C. Saturation density is reached at 1x 100,000 cells/cm². Cells may attach lightly and so handling should be kept to a minimum. Care shoul

기타 정보

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