h1RPE7

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human eye
• 포장: tube of 5 μg 09061602-DNA-5UG; pkg of vial of cells 09061602-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Polygonal
• 제품: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice

설명

세포주 기원

An immortalized retinal pigment epithelial cell line

세포주 설명

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

DNA 프로파일

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19

배양 배지

Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 24hrs.

기타 정보

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면책조항

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.