MEWO

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93082609, human skin, Fibroblast

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human skin
• 포장: tube of 5 μg 93082609-DNA-5UG; pkg of vial of cells 93082609-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Melanin
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human malignant melanoma

세포주 설명

Granular fibroblasts. Derived from a human melanoma by Prof. C Grose (1978). The cells support growth of varicella-zoster virus (VZV) isolates at 36°C although viral growth is optimal at 32°C.

애플리케이션

Virus studies

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA (0.5% trypsin in 0.53mM EDTA in modified Puck′s saline A); 5% CO₂; 37°C.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.