ND8/34 Cell Line from mouse

92090904, Mouse x Rat Hybridoma nerve, Neuronal

• UNSPSC 코드: 41106514

속성

• 제품명: ND8/34 Cell Line from mouse, 92090904
• 생물학적 소스: Mouse x Rat Hybridoma nerve
• 설명: Mouse neuroblastoma x Rat neurone hybrid
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Neuronal
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse neuroblastoma x Rat neurone hybrid

세포주 설명

The hybrid ND8/34 is a subclone of ND8 which was derived after fusion of primary neonatal rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. ND8/34 are electrically excitable to the chemical activator of nociceptive sensory neurons, bradykinin.

애플리케이션

Study of neuronal differentiation.

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

기타 정보

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