J774A.1 Cell Line from mouse

91051511, mouse blood, Not specified

• 동의어(들): J774 A1 Cells, J774.A1 Cells, J774A1 Cells
• UNSPSC 코드: 41106514

속성

• 제품명: J774A.1 Cell Line from mouse, 91051511
• 생물학적 소스: mouse blood
• 설명: Mouse BALB/c monocyte macrophage
• 성장 모드: Semi-adherent
• 핵형: Not specified
• 형태학: Not specified
• 제품: Interleukin 1 (IL-1), lysozyme
• 수용체: Immunoglobulin, complement
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse BALB/c monocyte macrophage

세포주 설명

Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.

애플리케이션

J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.

Metabolic studies

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm² Incubate at 37°C; 5% CO₂. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.

기타 정보

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