추천 제품
사용
sufficient for 40 labeling reactions
Quality Level
포장
kit of 1 (7 components)
제조업체/상표
Roche
환경친화적 대안 제품 특성
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
기술
hybridization: suitable
환경친화적 대안 카테고리
, Aligned
저장 온도
−20°C
일반 설명
DIG DNA Labeling Kit is a convenient kit for the labeling of DNA with Digoxigenin-deoxyuridine triphosphate (dUTP) using random oligonucleotides as primers. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
특이성
Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or by heating to 65 °C for 10 minutes.
애플리케이션
For Random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled DNA probes can be used for:
- All types of filter hybridization according to our standard protocol given in the pack insert of the special hybridization solution DIG Easy Hyb.
- Single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat.
- In situ hybridizations
포장
1 kit containing 7 components.
품질
Function tested in a Southern blot.
규격
Assay Time: Labeling: 1hour to O/N
Sensitivity and specificity: A single-copy human gene (tPA gene) is detected with a DIG-labeled probe in a Southern blot of 1μg digested human placenta DNA.
Sensitivity and specificity: A single-copy human gene (tPA gene) is detected with a DIG-labeled probe in a Southern blot of 1μg digested human placenta DNA.
원리
DIG-labeled DNA probes are generated according to the random-primed DNA labeling method which is based on the hybridization of random oligonucleotides to the denatured DNA template. The complementary DNA strand is synthesized by Klenow enzyme which uses the 3′-OH termini of the random oligonucleotides as primers and a mixture of deoxyribonucleotides containing DIG-11-dUTP, alkali-labile for elongation. This results in incorporation of digoxigenin into the newly synthesized DNA.
Note:
Note:
- The use of the alkali-labile form of DIG-11-dUTP enables easier and more efficient stripping of blots for rehybridization experiments with a second DIG-labeled probe.
- DNA probe, labeled with DIG-11-dUTP, alkali-labile must not be denatured using NaOH, but can be denatured by boiling in a waterbath.
기타 정보
For life science research only. Not for use in diagnostic procedures.
키트 구성품 전용
제품 번호
설명
- Unlabeled Control DNA 1 100 µg/ml
- Unlabeled Control DNA 2 100 µg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA 5.2 µg/ml
- Hexanucleotide Mix 10x concentrated
- dNTP Labeling Mixture 10x concentrated
- Klenow Enzyme, Labeling grade 2 U/µl
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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