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Merck
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11175041910

Roche

DIG Nucleic Acid Detection Kit

greener alternative

sufficient for 40 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization, suitable for Northern blotting

동의어(들):

DIG system

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About This Item

UNSPSC 코드:
41116134

사용

sufficient for 40 blots (10 cm x 10 cm each)

Quality Level

포장

kit of 1 (5 components)

제조업체/상표

Roche

환경친화적 대안 제품 특성

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

기술

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

환경친화적 대안 카테고리

저장 온도

−20°C

일반 설명

DIG Nucleic Acid Detection Kit is used for detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).
DIG Nucleic Acid Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

애플리케이션

DIG nucleic acid detection kit has been used in the confirmation of genetic manipulation.
The DIG Nucleic Acid Detection Kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
  • Southern blots
  • Northern blots
  • Other nucleic acid blotting applications
  • In situ hybridization applications
The kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
  • Southern blots
  • Northern blots
  • Other nucleic acid blotting applications
  • In situ hybridization applications

특징 및 장점

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

포장

1 kit containing 5 components.

원리

DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP antibody conjugate and the color substrates NBT and BCIP.

제조 메모

Working concentration: Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/ml
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution):
  • Anti-Digoxigenin-AP Conjugate (vial 3): 2 to 8 °C, it is stable for 12 months at this temperature
  • NBT/BCIP (vial 4): 2 to 8 °C, stable
    Note: During shipment of the kit on dry ice, a precipitate may occur which is dissolved by briefly warming to 37 °C
  • The blocking reagent (vial 5) is stable for 36 months and can be stored dry at 4 to 8 °C.
  • Autoclaved stock solution can be stored for several days to a week either unopened at 15 to 25 °C or at 4 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

키트 구성품 전용

제품 번호
설명

  • DIG-labeled Control DNA 5 µg/ml

  • DNA Dilution Buffer, [50 μg/ml] fish sperm DNA

  • Anti-Digoxigenin-AP Conjugate antibody 750 U/ml

  • NBT/BCIP, 50x stock solution 50x concentrated

  • Blocking Reagent

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

does not flashNot applicable

Flash Point (°C)

does not flashNot applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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