LL24

90073001, human lung, Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: LL24, 90073001
• 생물학적 소스: human lung
• 성장 모드: Adherent
• 핵형: 2n = 46; diploid
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human diploid lung

세포주 설명

Derived from normal tissue taken at post-mortem from the lung of a 5 year old male. An ampoule of cells frozen after one passage and estimated population doubling (PDL) 2 was submitted to the American Type Culture Collection in June, 1977. Derived populations at passage 6 and approximately 14 PDL are available for distribution. Progeny were found to undergo an additional 25 population doublings in longevity trials.

애플리케이션

Tumourigenicity studies

배양 배지

Ham′s F12 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, a further 20 population doublings expected.

기타 정보

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