HL60 Ast.3

88120801, human blood, Lymphoblastoid

• UNSPSC 코드: 41106514
• 생물학적 소스: human blood
• 성장 모드: Suspension
• 핵형: Modal no. = 93, Hypertetraploid
• 형태학: Lymphoblastoid
• 제품: Not specified
• 수용체: Not specified
• 관련 질환(들): cancer

속성

• 제품명: HL60 Ast.3, 88120801
• 생물학적 소스: human blood
• 성장 모드: Suspension
• 핵형: Modal no. = 93, Hypertetraploid
• 형태학: Lymphoblastoid
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian promyelocytic leukaemia

세포주 설명

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.

애플리케이션

Differentiation studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

계대배양 정규 작업

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic or the cell line will differentiate.

기타 정보

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