NCTC clone 1469

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88111403, mouse liver, Epithelial

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse liver
• 포장: tube of 5 μg 88111403-DNA-5UG; pkg of vial of cells 88111403-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse C3H/An liver

세포주 설명

Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

애플리케이션

Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)

배양 배지

NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO₂; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan

기타 정보

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