MNNG/HOS (TE85, Clone F-5)

87070201, human bone, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: MNNG/HOS (TE85, Clone F-5), 87070201
• 생물학적 소스: human bone
• 성장 모드: Adherent
• 핵형: Modal no. 70, 71; hypertriploid
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian osteosarcoma, chemically transformed

세포주 설명

A chemically transformed cell line (MNNG 0.01ug/ml) derived from an osteosarcoma of a 13 year female Caucasian. Grows to high saturation density and produces tumours in nude mice.

애플리케이션

Transformation and tumourigenicity studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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