BF-2

87032603, fish trunk, Fibroblast

• UNSPSC 코드: 41106514

속성

• 제품명: BF-2, 87032603
• 생물학적 소스: fish trunk
• 성장 모드: Adherent
• 핵형: 2n = 48
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Fish Bluegill fry caudal trunk

세포주 설명

Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15°C to 33°C. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.

애플리케이션

Virus studies, targets for bacterial cytotoxins

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (pH7.4 - 7.5).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin/EDTA; 5% CO₂; 23°C. A replication capacity of up to 50 passages is expected. Fish cell lines detach easily during transit if the culture is too young. After resuscitation, split cells

기타 정보

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