Ehrlich-Lettre Ascites strain E cell line

87032503, mouse unknown/unspecified, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: Ehrlich-Lettre Ascites strain E cell line, from mouse unknown/unspecified, 87032503
• 생물학적 소스: mouse unknown/unspecified
• 성장 모드: Adherent
• 핵형: 2n = 40
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse carcinoma

세포주 설명

Established from the explant of a 7 day old tumour from the parental Ehrlich-Lettre Ascites carcinoma. Prior to development, strain E was subjected to a schedule of seven mouse passages and six in vitro passages over a period of 17 months. The cells exhibit an unusually high mean chromosome number and possess the marker chromosomes present in the parental Ehrlich tumour. This cell line may be of use in comparative testing of anti-tumour agents in vivo and in vitro.

애플리케이션

Anti-tumour test and tumourigenicity studies

배양 배지

NCTC 135 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change 3 times a week.

기타 정보

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