AtT20

87021902, mouse brain, Small round cells growing as large aggregates

• UNSPSC 코드: 41106514

속성

• 제품명: AtT20, 87021902
• 생물학적 소스: mouse brain
• 포장: tube of 5 μg 87021902-DNA-5UG; pkg of vial of cells 87021902-1VL
• 성장 모드: Aggregates in suspension
• 핵형: Diploid
• 형태학: Small round cells growing as large aggregates
• 제품: Adrenocorticotropic hormone (ACTH)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse LAF1 pituitary gland tumour

세포주 설명

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

애플리케이션

Hormone studies

배양 배지

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

계대배양 정규 작업

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

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