CL 21

86080602, Human x Mouse Hybridoma unknown/unspecified, Fibroblast-like

• UNSPSC 코드: 41106514

속성

• 제품명: CL 21, 86080602
• 생물학적 소스: Human x Mouse Hybridoma unknown/unspecified
• 포장: tube of 5 μg 86080602-DNA-5UG; pkg of vial of cells 86080602-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse / Human hybrid, SV-40 transformed

세포주 설명

The cell line CL21 has been established by hybridisation of C57 BL mouse peritoneal macrophages with SV40 transformed human skin fibroblasts. The human cells were derived from a patient with Lesch-Nyhan syndrome and are deficient in hypoxanthine guanine phosphoribosyltransferase (HGPRT). They have been reported to contain the human chromosome 7 and could also contain subchromosomal fragments of chromosome 17.

애플리케이션

Somatic cell hybrid mapping

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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