A549 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012804, human lung (carcinoma), Epithelial

• 동의어(들): A-549 Cells, A549 Cells, NCI-A549 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human lung (carcinoma)
• 포장: tube of 5 μg 86012804-DNA-5UG; pkg of vial of cells 86012804-1VL
• 성장 모드: Adherent
• 핵형: Hypotriploid
• 형태학: Epithelial
• 제품: Lecithin, High amounts of de-saturated fatty acids.
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian lung carcinoma

세포주 설명

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

애플리케이션

Enzymatic studies

Human A549 cell line has been used:

• to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
• to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
• to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

배양 배지

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or tTrypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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