MCF7 Cell Line human

86012803, from human breast(adenocarcinoma)

• 동의어(들): IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
• UNSPSC 코드: 41106514
• eCl@ss: 32190102

속성

• 생물학적 소스: human breast (adenocarcinoma)
• 성장 모드: Adherent
• 핵형: 2n = 46, hypertriploid to hypotetraploid
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Reported to express the oestrogen and progesterone receptor
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian breast adenocarcinoma

세포주 설명

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

애플리케이션

Tumourigenicity and virus studies: Type B and C viruses

DNA 프로파일

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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