T47D Cell Line human

85102201, human breast, Epithelial

• 동의어(들): T-47-D Cells, T-47D Cells, T47D:A Cells
• UNSPSC 코드: 41106514

속성

• 제품명: T47D Cell Line human, 85102201
• 생물학적 소스: human breast
• 설명: Human breast tumor
• 성장 모드: Adherent
• 핵형: 2n = 46, hypertriploid, modal no. 65
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human breast tumour

세포주 설명

Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.

애플리케이션

T47D cell line human has been used:

• to study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans
• has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
• to study the anti-proliferative effects of two novel classes of potential antimetabolites on T47D cells

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.