BHK 21 (Clone 13) from hamster

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011433, hamster kidney, Fibroblast

• 동의어(들): BHK-21 (C-13) Cells, BHK-21 C-13 Cells, BHK21 clone 13 Cells, BHK21-C13 Cells, BHK21/C13 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: hamster kidney
• 포장: tube of 5 μg 85011433-DNA-5UG; pkg of vial of cells 85011433-1VL
• 성장 모드: Adherent
• 핵형: 2n = 44, pseudodiploid
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Hamster Syrian kidney

세포주 설명

Subclone of parent line derived from 5 1-day-old unsexed hamster kidneys. Used extensively for virus replication studies i.e. poliovirus, rabies, foot and mouth disease, VSV (Indiana strain), herpes simplex, Ad25 and arboviruses.

애플리케이션

BHK 21 (Clone 13) from hamster has been used in viral transfection studies.

Virus replication studies

배양 배지

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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