GPNT

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: rat brain
• 포장: tube of 5 μg 09061601-DNA-5UG; pkg of vial of cells 09061601-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fusiform
• 제품: GPNT cells express tight junction proteins
• 수용체: P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice

설명

세포주 기원

An immortalised Lewis rat brain vascular endothelial cell line

세포주 설명

GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.

DNA 프로파일

Not specified

배양 배지

Hams F10 + 10% Heat-inactivated FCS + 2 mM Glutamine + 2 ng/ml basic fibroblast growth factor + 80 μg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10μg/cm2 from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to a mMonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 10hrs.

기타 정보

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면책조항

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.