C6-2-3

8062528, rat brain, fibroblastic

• UNSPSC 코드: 41106514

속성

• 제품명: C6-2-3, 08062528
• 생물학적 소스: rat brain
• 성장 모드: Adherent
• 핵형: modal No varied with passage see reference
• 형태학: fibroblastic
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat glioma x rat glioma hybrid

세포주 설명

A pseudotetraploid call line generated by fusion of 8-bromodeoxyuridine-resistant mutant rat glioma cell line C6-BU-1 and the 6-thioguanine-resistant mutant rat glioma cell line C6-TG-1.

애플리케이션

electrophysiology

배양 배지

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C

기타 정보

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