ANGM-CSS

8040401, human brain, Fusiform, can form heaps of cells

• UNSPSC 코드: 41106514

속성

• product name: ANGM-CSS, 08040401
• 생물학적 소스: human brain
• 성장 모드: Not specified
• 핵형: Near tetraploid; range 88 to 91 - see Cell Line Description for more information
• 형태학: Fusiform, can form heaps of cells
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human glioblastoma

세포주 설명

A human A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present. (Information provided by Depositor)

배양 배지

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37ºC.

기타 정보

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