22Rv1

5092802, human prostate, Epithelial

• 동의어(들): 22Rv-1 Cells, CWR-22rv1 Cells, CWR22-R1 Cells
• UNSPSC 코드: 41106514

속성

• product name: 22Rv1, 05092802
• 생물학적 소스: human prostate
• 성장 모드: Adherent aggregates
• 핵형: 49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+12[5]/50,idem,+3[1]
• 형태학: Epithelial
• 제품: Not specified
• 수용체: androgen receptor positive
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Xenograft Prostate

세포주 설명

22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor β-1 (TGF β-1). This cell line is tumorigenic in nude mice.

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 15,21

배양 배지

RPMI 1640 without phenol red (Sigma R7509) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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