L929/R

04102001

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse adipose tissue
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse C3H/An connective tissue cisplatin resistant

세포주 설명

This cisplatin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (Sigma Catalogue number. 85011425) to increasing concentrations of cisplatin in vitro. L929/R cells can be used in the development of novel anti-cancer treatments. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

애플리케이션

Development of novel anti-cancer treatments

배양 배지

RPMI 1640 + 2mM L-Glutamine + 10% Foetal Bovine Serum (FBS) + 0.26μM Cisplatin.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:5 to 1:50 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 2% CO₂; 37 C.

기타 정보

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