C9903

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder, enterotoxin

• 동의어(들): CTB
• MDL number: MFCD02356522
• UNSPSC 코드: 12352200
• PubChem Substance ID: 329775212
• NACRES: NA.77

속성

• 제품명: Cholera Toxin B subunit, ≥95% (SDS-PAGE), lyophilized powder
• Quality Level: 300
• 분석: ≥95% (SDS-PAGE)
• 양식: lyophilized powder
• 분자량: ~12 kDa
• 구성: Protein, ~5% Lowry
• 불순물: ≤0.5% Cholera toxin A subunit (SDS-PAGE)
• solubility: H₂O: 10 mg/mL
• 저장 온도: 2-8°C
• SMILES string: CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
• InChI: 1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
• InChI key: YDXZSNHARVUYNM-UHFFFAOYSA-N

설명

일반 설명

Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.

애플리케이션

Cholera Toxin B subunit has been used:

• for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
• in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

생화학적/생리학적 작용

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

특징 및 장점

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

포장

Package size based on protein content

물리적 형태

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.

재구성

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN₃ and 1 mM sodium EDTA.

분석 메모

Activity measured by ELISA using ganglioside GM₁-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H312 + H332,H412
• 예방조치 성명서: P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable