추천 제품
형태
lyophilized powder
Quality Level
특이 활성도
≥25 units/mg protein
분자량
~260 kDa
구성
Protein, ≥15% biuret
저장 온도
−20°C
애플리케이션
Creatinine Deiminase microbial has been used:
- to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
- in creating creatinine-sensing membrane for biophysical studies
- to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples
Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.
생화학적/생리학적 작용
Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.
물리적 특성
Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
단위 정의
One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.
물리적 형태
Lyophilized powder containing mannitol as stabilizer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Anne K Bendt et al.
Archives of microbiology, 181(6), 443-450 (2004-05-19)
In order to utilize different nitrogen sources and to survive situations of nitrogen limitation, microorganisms have developed several mechanisms to adapt their metabolism to changes in the nitrogen supply. In this communication, the use of creatinine as an alternative nitrogen
Hybrid biosensors for clinical analysis and fermentation control.
I Karube
Methods in enzymology, 137, 131-138 (1988-01-01)
T W Esders et al.
The Journal of biological chemistry, 260(7), 3915-3922 (1985-04-10)
Creatinine iminohydrolase (EC 3.5.4.21), which catalyzes hydrolysis of creatinine to N-methylhydantoin and ammonia, was purified from Flavobacterium filamentosum. The average molecular weight of the purified enzyme was 272,480, and the subunit molecular weight was 44,300. Extensive specificity studies indicated that
[Immobilized enzyme reactors--their use in chemiluminescence detection].
M Tabata et al.
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 31(3), 220-229 (1986-03-01)
Hsiao-Chung Tsai et al.
Analytical biochemistry, 334(1), 183-192 (2004-10-07)
An optical array biosensor encapsulated with hydrolase and oxidoreductase using sol-gel immobilization technique has been fabricated for simultaneous analysis and screening of multiple samples to determine the presence of multianalytes which are clinically important in relation to renal failure. Urease
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