추천 제품
생물학적 소스
mouse
결합
CY3 conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
15B8, monoclonal
형태
buffered aqueous solution
분자량
antigen 94 kDa
종 반응성
chicken, bovine, human, canine
농도
~1 mg/mL
기술
direct immunofluorescence: 1:100 using methanol/acetone-fixed bovine MDBK/MCF-7 Cell Line
동형
IgG1
UniProt 수납 번호
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... CTNNB1(1499)
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일반 설명
Anti-β-Catenin-Cy3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant chicken catenin. β-catenin is 92 kDa. β-catenin protein is composed of a series of protein-protein interaction motifs that allow it to function as a scaffold. The N-terminus domain contains the binding site for β-catenin as well as phosphorylation sites recognized by Glycogen synthase kinase 3 beta (GSK3β) and the C-terminus contains the transcriptional activation domain and binding site for Teashirt.
The gene β-catenin (CTNNB1) is mapped to human chromosome 3p21. The protein localizes to the cytoplasm and the nucleus. β-catenin is also present at the centrosomes.
특이성
Recognizes specifically mammalian and avian β-catenin. Does not cross-react with plakoglobin (γ-catenin).
면역원
recombinant chicken β-catenin.
애플리케이션
Anti-β-Catenin-Cy3 antibody has been used in
- direct immunofluorescence
- western blotting
- staining
- immunohistochemistry
Suitable for direct immunofluorescence at a dilution of 1:100 using methanol/acetone-fixed bovine MDBK/MCF-7 cells. The antiobody has been used for western blot analysis in mouse embryonic stem cells.
생화학적/생리학적 작용
Catenin (cadherin-associated protein) β 1 also called β-catenin is encoded by the CTNNB1 gene in humans. It is a dual function protein that regulates the coordination of cell-cell adhesion and gene transcription. It is useful for differential diagnosis of osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and provide a potential target for CCA therapy. Mutations in β-catenin are associated with ovarian carcinoma. In addition, mutations in β-catenin are linked to intellectual disability. NEK2 (Never in mitosis A-related kinase 2) binds and phosphorylates β-catenin, thereby regulating centrosome seperation. Focal adhesion protein, Kindlin-2, enhances WNT signaling by forming a transcriptional complex with β-catenin.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
법적 정보
Cy is distributed under license from Amersham Biosciences Limited.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Alma Kuechler et al.
Human genetics, 134(1), 97-109 (2014-10-20)
Recently, de novo heterozygous loss-of-function mutations in beta-catenin (CTNNB1) were described for the first time in four individuals with intellectual disability (ID), microcephaly, limited speech and (progressive) spasticity, and functional consequences of CTNNB1 deficiency were characterized in a mouse model.
Self-regulation of functional pathways by motifs inside the disordered tails of beta-catenin
Zhao B and Xue B
BMC Genomics, 17(5), 484-484 (2016)
Tissue factor and thrombomodulin expression on endothelial cell-seeded collagen modules for tissue engineering
She M, et al.
Journal of Biomedical Materials Research Part A, 80(2), 497-504 (2007)
Yang Wan et al.
Human pathology, 45(7), 1459-1465 (2014-04-22)
Osteoblastoma (OB) and osteosarcoma (OS) are 2 bone tumors that predominantly affect young adults. The clinical management of OS differs significantly from that of OB, and thus, accurate diagnosis of OB and OS is critical in determining appropriate treatment modality.
Shirin Bahmanyar et al.
Genes & development, 22(1), 91-105 (2007-12-19)
beta-Catenin plays important roles in cell adhesion and gene transcription, and has been shown recently to be essential for the establishment of a bipolar mitotic spindle. Here we show that beta-catenin is a component of interphase centrosomes and that stabilization
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