콘텐츠로 건너뛰기
Merck
모든 사진(1)

Key Documents

C6974

Sigma-Aldrich

Anti-CaM Kinase IIα (CaMKIIα) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

로그인조직 및 계약 가격 보기


About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.44

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

IgG fraction of antiserum

항체 생산 유형

primary antibodies

클론

polyclonal

형태

buffered aqueous solution

분자량

antigen 50 kDa

종 반응성

human, chicken, rat, mouse

포장

antibody small pack of 25 μL

기술

microarray: suitable
western blot: 1:5,000 using synaptosomal fraction of rat brain

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... CAMK2A(815)
mouse ... Camk2a(12322)
rat ... Camk2a(25400)

일반 설명

Ca2+/Calmodulin dependent protein kinase II (CaMKII) belongs to the family of Ser/Thr protein kinases including CaMKI and CaMKIV. CaM kinase II is one of the most abundant protein kinases in the mammalian brain, with the highest expression in neurons of the hippocampus and the cerebral cortex. CaMKII consists of a family of four related isoforms CaMK IIα, CaMK IIβ, CaMK IIγ and CaMK IIδ. The CaMK II α and β isoforms are predominantly expressed in the brain, localized mainly in the cytosol and postsynaptic densities (PSDs), whereas the CaMKII γ and δ isoforms are expressed in all tissues. CaMKII contains a catalytic and regulatory domain. The regulatory domain consists of an autoinhibitory and calmodulin binding site.

특이성

The antibody does not cross-react with CaMKIIβ, γ and δ isoforms (60 kDa). Anti-CaM Kinase IIα specifically binds to CaM Kinase IIα (50kDa) in human, mouse, rat and chicken. CaM Kinase IIα immunizing peptide specifically inhibits the staining of CaM Kinase IIα in immunoblotting.

면역원

Peptide corresponding to amino acids 461-478 of rat CaM Kinase Iiα, conjugated to KLH. This sequence is identical in human, mouse and chicken CaMKIIα has limited homology (50-60%) with CaM Kinase IIβ, γ and δ subunits.

애플리케이션

Anti-CaM Kinase IIα (CaMKIIα) antibody has been used:
  • in immunolabelling
  • in western blotting
  • in immunoprecipitation
  • in immunoblotting

생화학적/생리학적 작용

CaMKII is involved in many cellular functions in response to Ca2+ signaling, including synthesis and secretion of neurotransmitters, axonal transport, long term potentiation (LTP) and spatial learning, receptor function and regulation of gene expression. CaMKII plays a critical role in LTP, a cellular model of learning and memory. In the CNS, postsynaptic Ca2+ influx triggers rapid autophosphorylation and stable activation of CaMKII in a Ca2+/calmodulin dependent manner at a threonine residue in the autoinhibitory domain. Autophosphorylation of CaMKIIα at Thr286 has been shown to be required for LTP and learning.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Martin W Berchtold et al.
The Journal of biological chemistry, 291(52), 26636-26646 (2016-11-07)
Calmodulin (CaM) is a Ca
Tianzhi Huang et al.
Nature communications, 7, 12885-12885 (2016-10-05)
Molecularly defined subclassification is associated with phenotypic malignancy of glioblastoma (GBM). However, current understanding of the molecular basis of subclass conversion that is often involved in GBM recurrence remain rudimentary at best. Here we report that canonical Wnt signalling that
Structure of the autoinhibited kinase domain of CaMKII and SAXS analysis of the holoenzyme
Rosenberg O S, et al.
Cell, 123(5), 849-860 (2005)
CaMKII beta functions as an F-actin targeting module that localizes CaMKII alpha beta heterooligomers to dendritic spines
Shen K, et al.
Neuron, 21(3), 593-606 (1998)
Yu-Chih Lin et al.
Communicative & integrative biology, 2(1), 40-41 (2009-08-26)
CaMKII, calcium/calmodulin dependent protein kinase, is an active kinase in the cell that phosphorylates a number of substrates including several cytoskeletal and signaling proteins. In addition to kinase activity, the beta isoform of CaMKII also contains an F-actin binding region.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.