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Key Documents

C6788

Sigma-Aldrich

4-Chloro-1-naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

동의어(들):

4-CN, 4-chloronaphthalen-1-ol

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About This Item

Linear Formula:
ClC10H6OH
CAS Number:
Molecular Weight:
178.61
Beilstein:
509750
EC Number:
MDL number:
UNSPSC 코드:
12352204
PubChem Substance ID:
NACRES:
NA.83

product name

4-Chloro-1-naphthol, tablet

형태

tablet

Quality Level

mp

118-121 °C (lit.)

solubility

methanol: 10 mL, clear, colorless

저장 온도

−20°C

SMILES string

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI key

LVSPDZAGCBEQAV-UHFFFAOYSA-N

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특이성

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

애플리케이션

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

수량

Contains 30 mg substrate per tablet.

재구성

Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


시험 성적서(COA)

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문서

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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