C4249

Cystatin B human

recombinant, expressed in E. coli, solid, ≥95% (SDS-PAGE)

• 동의어(들): CPI-B, CST6, CSTB, EPM1, Liver Thiol Proteinase Inhibitor, PME, STFB, Stefin B
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Cystatin B human, recombinant, expressed in E. coli
• 재조합: expressed in E. coli
• Quality Level: 200
• 설명: contains a N-terminal 7X His-tag
• 분석: ≥95% (SDS-PAGE)
• 형태: solid
• 분자량: apparent mol wt ~13 kDa by SDS-PAGE (reducing)
• NCBI 수납 번호: NP_000091
• 저장 온도: −20°C
• 유전자 정보: human ... CSTB(1476)

설명

일반 설명

Cystatin B is a member of the type 1 (stefins) superfamily of cystatins. Cystatin B is encoded by the CSTB gene and functions as an intracellular thiol protease inhibitor regulating the activity of members of the papain family and cathepsins B, H, and L. Cystatin B may also protect cellular components from the effects of proteases that leak from lysosomes. Mutations in the CSTB gene are thought to be the primary defect in patients with Progressive Myoclonus Epilepsy (EPM1).

애플리케이션

Suitable for use as a cysteine-protease inhibitor. Is tested for suitability as an inhibitor of the enzymatic activity of papain.

생화학적/생리학적 작용

Cystatin B was shown to interact with the protein RACK-1, β-spectrin and neurofilament light chain NF-L forming a unique multiprotein complex specifically in the cerebellum, confirming its function in the brain.

재구성

Cystatin B should be reconstituted at 100 μg/mL in steile 25 mM Tris pH 7.5 and 100 mM NaCl. Reconstituted product should be stored in single-use aliquots at -70° C and should be stable for at least 3 months.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable