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Merck
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주요 문서

C-28057

Sigma-Aldrich

Macrophage Base Medium DXF

Basal Medium, 250 ml

동의어(들):

Macrophage Culture Medium

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

양식

liquid

포장

pkg of 250 ml

기술

cell culture | mammalian: suitable

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Macrophage Base Medium DXF (supplements sold separately)

애플리케이션

The Macrophage Base Medium DXF is the user-customizable version of the Macrophage Generation Media DXF that does not include cytokines. It must be adequately supplemented by the user for the generation of monocyte-derived Macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed. Recommended for:Human Mononuclear Cells (hMNC), freshly isolatedHuman Monocytes (hMo), freshly isolated

성분

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

품질

All lots of PromoCell Macrophage Media DXF are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell Macrophage Media DXF is tested for the ability to support Macrophage differentiation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

저장 및 안정성

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

계대배양 정규 작업

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Storage Class Code

10 - Combustible liquids


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시험 성적서(COA)

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문서

Cryopreserved blood cells and cell culture media for CD34+ human hematopoietic progenitor stem cells, monocytes, PBMCs, macrophages, dendritic and T-cells. Explore over 350 PromoCell products on Sigma.com.

프로토콜

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

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