C2181

Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment–Cy3 antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Cy3 Anti-Mouse IgG, Cy3 Mouse IgG, Sheep Anti-Mouse IgG
• MDL number: MFCD02179333
• UNSPSC 코드: 12352203
• NACRES: NA.46

속성

• 생물학적 소스: sheep
• 결합: CY3 conjugate
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: secondary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 종 반응성: mouse
• 기술: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Igs have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

애플리케이션

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Cy3 antibody produced in sheep was used for BrdU staining of frozen quail muscle cross sections at a dilution of 1:200 to analyze activated, proliferating muscle precursor cells.

Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment−Cy3 antibody produced in sheep has been used:

• in immunolabeling of Hela cells
• as secondary antibody in immunocytochemistry of dendritic cells
• as secondary antibody in immunofluorescence analysis of mesenchymal stem cells
• as secondary antibody in immunofluorescence staining keratinocyte cell lines
• in immunohistochemistry of articular cartilage

The product binds to all mouse Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.

생화학적/생리학적 작용

Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Mouse IgG (whole molecule) F(ab′)2 fragment antibody allows for the visualization of protein by fluorescent microscopy.

기타 정보

Antibody adsorbed with human serum proteins.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

제조 메모

Adsorbed to reduce background staining with human samples.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable