C1261

Carboxypeptidase A−Agarose

ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

• 동의어(들): Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
• 효소 위원회 번호: 3.4.17.1 (BRENDA, IUBMB)
• MDL number: MFCD00130718
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: enzyme from bovine pancreas
• Quality Level: 200
• 양식: ammonium sulfate suspension
• 특이 활성도: ≥6 units/mL packed gel, 25 °C
• 분자량: ~35,250
• Matrix: beaded agarose
• 저장 온도: 2-8°C

설명

일반 설명

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

생화학적/생리학적 작용

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.

Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.

단위 정의

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

물리적 형태

Suspension in 2.0 M (NH₄)₂SO₄, pH 7

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable