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Merck
모든 사진(1)

주요 문서

C0858

Sigma-Aldrich

Cyclooxygenase 2 human

≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein

동의어(들):

COX-2, Prostaglandin H synthase 2

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About This Item

MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.32

재조합

expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells.
expressed in insect cells

Quality Level

분석

≥70% (SDS-PAGE)

양식

aqueous solution

특이 활성도

≥8000 units/mg protein

분자량

dimer subunit mol wt 70-74 kDa

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

human ... PTGS2(5743)

생화학적/생리학적 작용

COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.

기타 정보

Tagged with a six-residue histidine sequence near the N-terminus.

단위 정의

One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.

물리적 형태

Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN® 20 and 10% glycerol

기질

KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

법적 정보

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

María J Pérez et al.
Journal of food science, 83(5), 1454-1462 (2018-04-17)
This work is part of the search in native food matrices from arid regions of Argentina of interest to improve human health. Prosopis species are ethnic food resources in South America capable of growing in arid and semi-arid environments. This
Masanori Sugimoto et al.
Drug development research, 77(1), 20-28 (2016-01-15)
Preclinical Research This article describes the properties of a novel topical NSAID (Nonsteroidal anti-inflammatory drug) patch, SFPP (S(+)-flurbiprofen plaster), containing the potent cyclooxygenase (COX) inhibitor, S(+)-flurbiprofen (SFP). The present studies were conducted to confirm human COX inhibition and absorption of
Alejandra Moreno et al.
Natural product communications, 10(6), 991-994 (2015-07-23)
Zuccagnia punctata Cav. (Fabaceae) is an Argentine medicinal aromatic shrub (jarilla pispito, puspus, lata and jarilla macho). The chalcones were identified as pigments responsible for the yellow color of the flowers. Hydroethanolic extracts were obtained both from fresh flowers and
Kaneez Fatima et al.
Frontiers in nutrition, 9, 857256-857256 (2022-04-26)
Carvacrol, a monoterpene known for its pharmacological activities, is present in the essential oil of Origanum majorana, Origanum vulgare, Thymus vulgaris, and Lippia graveolens. It is used in food as a flavoring and preservative agent in cosmetics and medicines because
Tetsuo Kida et al.
PloS one, 9(5), e96481-e96481 (2014-05-07)
To evaluate the pharmacokinetics and efficacy of topically applied nonsteroidal anti-inflammatory drugs (NSAIDs) in the retinochoroidal tissues of rabbits. The cyclooxygenase (COX) inhibitory activity of diclofenac, bromfenac, and amfenac, an active metabolite of nepafenac, were determined using human-derived COX-1 and

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