형광
λex 546 nm; λem 579 nm
배송 상태
wet ice
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
The Baseclick EdU In Vivo Kits provide a superior alternative to BrdU and [3H]thymidine assays for detection of cell proliferation in whole animals, showing no toxic effects. EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU In Vivo Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU In Vivo Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.The in vivo kits are designed in a sense that you combine one of the three baseclick EdU cell proliferation kits such as:
1. Imaging kit (BCK-IV-IM)
2. Flow cytometry kit (BCK-IV-FC)
3. High throughput screening – HTS kit (BCK-IV-HTS)
You can select the above kits with the dye of choice and the right EdU content for your animal model.
Depending on your animal model or the number of animals to be tested you can choose between three kit sizes S, M, and L with increasing EdU content, as indicated in the following table.
Kit Size Content of EdU
S 50 mg
M 500 mg
L 1000 mg
1. Imaging kit (BCK-IV-IM)
2. Flow cytometry kit (BCK-IV-FC)
3. High throughput screening – HTS kit (BCK-IV-HTS)
You can select the above kits with the dye of choice and the right EdU content for your animal model.
Depending on your animal model or the number of animals to be tested you can choose between three kit sizes S, M, and L with increasing EdU content, as indicated in the following table.
Kit Size Content of EdU
S 50 mg
M 500 mg
L 1000 mg
애플리케이션
in Vivo, High Throughput Screening
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Corr. 1C
보충제 위험성
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Maia Dorsett et al.
Genetics, 183(1), 233-247 (2009-07-15)
Germ-line stem cells are unique because they either self-renew through mitosis or, at a certain frequency, switch to meiosis and produce gametes. The switch from proliferation to meiosis is tightly regulated, and aberrations in switching result in either too little
Adrian Salic et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(7), 2415-2420 (2008-02-15)
We have developed a method to detect DNA synthesis in proliferating cells, based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction ("click" chemistry). Detection of the
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