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Merck
모든 사진(4)

주요 문서

B9410

Sigma-Aldrich

BIS-TRIS propane

BioXtra, ≥99.0% (titration)

동의어(들):

1,3-Bis[tris(hydroxymethyl)methylamino]propane

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About This Item

Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
Molecular Weight:
282.33
Beilstein:
1786109
EC Number:
MDL number:
UNSPSC 코드:
12161700
PubChem Substance ID:
NACRES:
NA.25

제품 라인

BioXtra

Quality Level

분석

≥99.0% (titration)

양식

powder

불순물

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

무기 잔류물

≤0.1%

pH

10.0-12.0 (1 M in H2O)

유용한 pH 범위

6.3-9.5

pKa(25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

solubility

H2O: 1 M, clear, colorless

음이온 미량물

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

양이온 미량물

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%

흡수

≤0.05 at 280 in H2O at 1 M
≤0.1 at 260 in H2O at 1 M

응용 분야

diagnostic assay manufacturing

저장 온도

room temp

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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애플리케이션

  • A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole Blood.: BIS-TRIS propane is used as a buffering agent in the capillary electrophoresis method to assess global DNA methylation levels in human whole blood. The buffer′s role is crucial in maintaining the stability and reproducibility of the electrophoretic conditions (Zinellu et al., 2019).

  • Low potential manganese ions as efficient electron donors in native anoxygenic bacteria.: BIS-TRIS propane is used to buffer the experimental conditions in studying the role of low potential manganese ions as electron donors in bacteria. The findings enhance our understanding of electron transfer processes in microbial systems (Deshmukh et al., 2018).

  • Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection.: This research uses BIS-TRIS propane in a capillary electrophoresis method to evaluate DNA methylation levels. The buffer′s properties ensure accurate and reliable detection, which is crucial for epigenetic studies (Zinellu et al., 2017).

기타 정보

Easily compare specifications for BIS-TRIS propane products with the BIS-TRIS propane specification table.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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이미 열람한 고객

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Hamed Tahmasbi et al.
Journal of pharmaceutical and biomedical analysis, 171, 1-7 (2019-04-07)
Secreted recombinant activated clotting factor VII activated (rFVIIa) in cell culture media missing gamma-carboxyglutamic acid (Gla) domain as a result of failure in gamma-carboxylation or cell lysis is called Gla-domainless impurity which has less negative charge compared to native rFVIIa.
Joseph S Brunzelle et al.
Archives of biochemistry and biophysics, 474(1), 157-166 (2008-04-01)
The three-dimensional structure of the catalytically efficient beta-xylosidase from Selenomonas ruminantium in complex with competitive inhibitor 1,3-bis[tris(hydroxymethyl)methylamino]propane (BTP) was determined by using X-ray crystallography (1.3A resolution). Most H bonds between inhibitor and protein occur within subsite -1, including one between
Sami J O Varjo et al.
Journal of chromatography. A, 1081(1), 92-98 (2005-07-15)
Greater stability of liposome coatings and improved resolution of model steroids in capillary electrochromatography (CEC) were sought by adding small diamines (ethylenediamine, diaminopropane, bis-tris-propane, or N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid, HEPES)) to the liposome solution before coating of fused silica capillaries. The phospholipid
Sarita V Mello et al.
Biomacromolecules, 4(4), 968-973 (2003-07-15)
In this paper, we describe the preparation and characterization of Langmuir and Langmuir-Blodgett (LB) monolayers of the enzyme organophosphorus acid anhydrolase (OPAA). Langmuir films of OPAA were characterized on different subphases, such as phosphate, ammonium carbonate, and bis-tris-propane buffers. Monolayers
P Gómez-Tagle et al.
Inorganic chemistry, 40(15), 3786-3796 (2001-07-10)
Potentiometric titrations of the mixtures of lanthanide(III) perchlorates and bis-Tris propane (BTP) reveal formation of dinuclear hydroxo complexes M2(BTP)2(OH)n(6-n), where M = La(III), Pr(III), Nd(III), Eu(III), Gd(III), and Dy(III) and n = 2, 4, 5, or 6, in the pH

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