B2904
Bluo-Gal
reagent for selection of recombinant bacterial clones
동의어(들):
5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
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모든 사진(3)
About This Item
실험식(Hill 표기법):
C14H16BrNO6
CAS Number:
Molecular Weight:
374.18
Beilstein:
1550140
MDL number:
UNSPSC 코드:
12352200
PubChem Substance ID:
NACRES:
NA.85
추천 제품
Grade
for molecular biology
무균
non-sterile
분석
≥98% (HPLC)
양식
powder
기술
nucleic acid detection: suitable
solubility
DMF: soluble
적합성
suitable for substrate for β-galactosidase
저장 온도
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
InChI key
LINMATFDVHBYOS-MBJXGIAVSA-N
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일반 설명
Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
애플리케이션
Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.
원리
When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
관련 제품
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
Lot/Batch Number
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