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Merck
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Key Documents

A7979

Sigma-Aldrich

Bovine Serum Albumin solution

35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

동의어(들):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.75

생물학적 소스

bovine

Quality Level

설명

endotoxin tested

무균

sterile-filtered

제품 라인

BioXtra

분석

33-37% total protein basis (UV)

형태

liquid

기원

USA origin

농도

35% in DPBS

기술

blocking: suitable
cell culture | mammalian: suitable

불순물

≤1000 EU/mL Endotoxin level

pH

6.5-7.5

외래 활성

Mycoplasma Test by Barile On raw material, none detected

배송 상태

ambient

저장 온도

2-8°C

일반 설명

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

애플리케이션

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

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문서 라이브러리 방문

Neil T Burford et al.
Assay and drug development technologies, 15(7), 320-329 (2017-11-10)
Homogeneous functional assays that utilize competition binding technology are widely used for determining pharmacological properties such as intrinsic activity and potency. One example is time-resolved fluorescence resonance energy transfer (TR-FRET) 3',5'-cyclic adenosine monophosphate (cAMP) assays, where labeled cAMP (tracer) and
Gustavo A Gomez et al.
Developmental biology, 449(2), 99-106 (2019-03-04)
The developmental biology of neural crest cells in humans remains unexplored due to technical and ethical challenges. The use of pluripotent stem cells to model human neural crest development has gained momentum. We recently introduced a rapid chemically defined approach
Brett S Peterson et al.
Cell reports, 24(1), 209-223 (2018-07-05)
SIRT3 is a nicotinamide adenine dinucleotide (NAD+)-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Fuel-stimulated insulin secretion, which involves mitochondrial metabolism, could be susceptible to SIRT3-mediated effects. We used CRISPR/Cas9 technology to manipulate SIRT3 expression in
John P Ray et al.
Nature communications, 11(1), 1237-1237 (2020-03-08)
Genome-wide association studies have associated thousands of genetic variants with complex traits and diseases, but pinpointing the causal variant(s) among those in tight linkage disequilibrium with each associated variant remains a major challenge. Here, we use seven experimental assays to
Bryne Ulmschneider et al.
The Journal of cell biology, 215(3), 345-355 (2016-11-09)
Despite extensive knowledge about the transcriptional regulation of stem cell differentiation, less is known about the role of dynamic cytosolic cues. We report that an increase in intracellular pH (pHi) is necessary for the efficient differentiation of Drosophila adult follicle

문서

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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