A6680

N-Acetylneuraminic Acid Aldolase from microorganisms

lyophilized powder, ≥20 units/mg protein (biuret)

• 동의어(들): N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase
• CAS Number: 9027-60-5
• 효소 위원회 번호: 4.1.3.3 (BRENDA, IUBMB)
• Beilstein: 2697172
• MDL number: MFCD00131656
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥20 units/mg protein (biuret)
• 분자량: ~98 kDa
• 저장 온도: −20°C

설명

애플리케이션

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.

Used in the Sialic Acid Quantification Kit, SIALIC-Q

물리적 특성

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾³M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg⁺⁺, Ag⁺
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)

단위 정의

One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C.

물리적 형태

Lyophilized powder containing mannitol and EDTA

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)