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Merck
모든 사진(3)

Key Documents

A6338

Sigma-Aldrich

Aldehyde Dehydrogenase, potassium-activated from baker′s yeast (S. cerevisiae)

lyophilized powder, ≥2.0 units/mg protein

동의어(들):

Aldehyde:NAD[P]+ oxidoreductase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

형태

lyophilized powder

Quality Level

특이 활성도

≥2.0 units/mg protein

분자량

228 kDa

구성

Protein, ≥5.0% biuret

배송 상태

dry ice

저장 온도

−20°C

일반 설명

Aldehyde dehydrogenase is a tetramer and has several different isoforms. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate. Potassium ions and cysteine are essential for the enzyme′s activity. Rubidium or NH4+ can be substituted for K+, and glutathione for cysteine. Lithium, Na+, and Cs+ inhibit the reaction. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme may be utilized to quantitate aldehydes present in blood.
Aldehyde dehydrogenase (ALDH) is present in the nucleus, cytosol, mitochondria and endoplasmic reticulum of cells.

애플리케이션

Aldehyde dehydrogenase (ALDH) has been used to evaluate the effects of pear extracts on ALDH activity. It has also been used to colorimetrically determine ethanol by monitoring the enzymatic reduction of nicotinamide adenine dinucleotide (NAD).

생화학적/생리학적 작용

Aldehyde dehydrogenase from baker′s yeast catalyzes the reduction of pyridine nucleotides by several aldehydes. It catalyzes the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. Aldehyde dehydrogenase is used to study the production of ethanol and isobutanol. Ethanol concentration can be determined colorimentrically by monitoring the enzymatic reduction of nicotinamide adenine dinucleotide (NAD) using alcohol dehydrogenase after preremoval of aldehyde by aldehyde dehydrogenase.

단위 정의

One unit will oxidize 1.0 μmole of acetaldehyde to acetic acid per min at 25 °C at pH 8.0 in the presence of β-NAD+, potassium and thiols.

물리적 형태

Contains lactose, potassium phosphate and citrate buffer salts, and mercaptosuccinic acid.

재구성

This enzyme can be dissolved at 0.3 mg/mL in 100 mM Tris-HCl buffer (pH 8.0), containing 0.02% BSA.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서 라이브러리 방문

Enzymic determination of acetaldehyde in blood.
F LUNDQUIST
The Biochemical journal, 68(1), 172-177 (1958-01-01)
Monoamine oxidase A activity in fibroblasts as a functional confirmation of MAOA variants.
Tessa M A Peters et al.
JIMD reports, 58(1), 114-121 (2021-03-18)
A two-stage, two-organism process for biohydrogen from glucose
Redwood MD and Macaskie LE
International Journal of Hydrogen Energy, 31(11), 1514-1521 (2006)
Sergey Stolyar et al.
Molecular systems biology, 3, 92-92 (2007-03-14)
The rate of production of methane in many environments depends upon mutualistic interactions between sulfate-reducing bacteria and methanogens. To enhance our understanding of these relationships, we took advantage of the fully sequenced genomes of Desulfovibrio vulgaris and Methanococcus maripaludis to
Ho-Sun Lee et al.
Phytotherapy research : PTR, 26(11), 1753-1758 (2012-03-28)
Korean pear (Pyrus pyrifolia cv. Shingo) has been used as a traditional medicine for alleviating alcohol hangover. However, scientific evidence for its effectiveness or mechanism is not clearly established. To investigate its mechanism of alcohol detoxification, both in vitro and

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