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Merck
모든 사진(1)

주요 문서

A3151

Sigma-Aldrich

Avidin–Peroxidase

lyophilized powder

동의어(들):

Avidin-POD

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

avidin from egg white
enzyme from horseradish

Quality Level

결합

peroxidase conjugate

양식

lyophilized powder

구성

Protein, ≥70% E1%/280

라벨링 범위

0.7-2.0 mol peroxidase per mol avidin

기술

direct ELISA: 1:50,000

solubility

H2O: soluble 1 mg/mL
PBS: soluble

저장 온도

−20°C

관련 카테고리

일반 설명

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

애플리케이션

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG1.
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

물리적 형태

Lyophilized powder containing citrate buffer.

제조 메모

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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