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Merck
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A2917

Sigma-Aldrich

Acrylamide/Bis-acrylamide

for molecular biology, BioReagent, 19:1 (ratio)

동의어(들):

Acrylamide/Bisacrylamide, 19:1 (5% crosslinker), Polyacrylamide solution

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About This Item

MDL number:
MFCD00080848
UNSPSC 코드:
41105319
PubChem Substance ID:
329770804
NACRES:
NA.25

Grade

for molecular biology

제품 라인

BioReagent

형태

powder

피드(feed) 비율

19:1 (ratio)

외래 활성

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

애플리케이션

Acrylamide/Bis-acrylamide has been used:
  • in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP)
  • in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotyping
  • in polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

특징 및 장점

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

재구성

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.
To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

픽토그램

Skull and crossbonesHealth hazard
GHS06,GHS08

신호어

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

표적 기관

Peripheral nervous system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Congenital Heart Disease, 129-156 (2006)
Preparation of sequencing RNA libraries through chemical Cross-linking coupled to affinity purification (cCLAP) in Saccharomyces cerevisiae
Wang C, et al.
Bio-protocol, 8(19) (2018)
Estradiol via estrogen receptor beta influences ROS levels through the transcriptional regulation of SIRT3 in human seminoma TCam-2 cells
Panza S, et al.
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 39(5), 1-8 (2017)
Ana Techniqs in Biotechnology (2011)
Preparation and properties of acrylamide gel for use in electrophoresis.
Raymond S and Wang YJ
Analytical Biochemistry, 1(4-5), 391-396 (1960)
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