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Merck
모든 사진(1)

주요 문서

A2831

Sigma-Aldrich

Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

동의어(들):

Anti-Dinitrophenyl, Monoclonal Anti-DNP

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

mouse

Quality Level

결합

alkaline phosphatase conjugate

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

SPE-7, monoclonal

양식

buffered aqueous glycerol solution

기술

direct ELISA: 1:25,000 using DNP-BSA
dot blot: 1:180,000 (chemiluminescence)

동형

IgE

배송 상태

wet ice

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

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일반 설명

Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.

면역원

DNP-KLH conjugate

애플리케이션

Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.
Used to induce controlled allergic reactions or as a source of mouse IgE.

물리적 형태

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 50% glycerol and 15 mM Sodium azide

분석 메모

Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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문서 라이브러리 방문

Urszula Krasuska et al.
Frontiers in plant science, 7, 840-840 (2016-07-06)
Canavanine (CAN), a structural analog of arginine (Arg), is used as a selective inhibitor of inducible NOS in mammals. CAN is incorporated into proteins' structure in the place of Arg, leading to the formation of aberrant compounds. This non-protein amino
B Jasani et al.
Journal of immunological methods, 150(1-2), 193-198 (1992-06-24)
Over the past 10 years an immunoperoxidase method using dinitrophenyl (DNP) hapten-labelled primary or secondary probes has been devised. Its widely successful application in research and diagnostic work has depended upon the development of certain key reagents. These include a

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