추천 제품
생물학적 소스
human cervix
Quality Level
형태
liquid
성장 모드
Adherent
핵형
2n = 46
형태학
Epithelial
제품
Not specified
수용체
Not specified
기술
cell culture | mammalian: suitable
관련 질환(들)
cancer
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Human cervix carcinoma
세포주 설명
These cells were originally Chang conjunctival cells adapted by Wong and Kilbourne, and designated as conjunctival ′D′ cells. Susceptible to swine influenza viruses, supports production of plaque formation by a variety of influenza virus subtypes. Has HeLa markers, exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Epithelial with occasional giant cells. Ethnicity: Black.
애플리케이션
Plaque assays, cloning, genetic and virus studies: swine influenza viruses
배양 배지
Medium 199 (HBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
계대배양 정규 작업
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using EDTA or 0.25% trypsin; 5% CO2; 37°C.
기타 정보
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면책조항
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
K S Lavappa
In vitro, 14(5), 469-475 (1978-05-01)
Seed stocks of human cell lines deposited at the American Type Culture Collection (ATCC) have been examined for cross-contamination with HeLa cells using Giemsabanded marker chromosomes. Sixteen additional cell lines investigated have been found to exhibit marker chromosomes typical of
Continuous subcultivation of epithelial-like cells from normal human tissues.
R S M CHANG
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 87(2), 440-443 (1954-11-01)
Examination of ATCC stocks for HeLa marker chromosomes in human cell lines.
K S Lavappa et al.
Nature, 259(5540), 211-213 (1976-01-22)
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