79454

Ampliflu^™ Red Western Blot Kit

for fluorescent detection

• UNSPSC 코드: 12352200

속성

• 사용: sufficient for 50 applications
• 형광: λ_ex 571 nm; λ_em 585 nm
• 저장 온도: 2-8°C

설명

일반 설명

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애플리케이션

The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.

원리

The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.

분석 메모

Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.

법적 정보

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

키트 구성품 전용

• Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC) 3 mL

키트 구성품 역시 별도로 이용 가능함

• 90101 Ampliflu^™ Red, for fluorescence, ≥98.0% (HPLC) 5 vial(s)
• 79382 Phosphate buffered saline, tablet 5 tablets