79390

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

• 동의어(들): Alkaline Phosphatase from calf intestinal mucosa
• CAS Number: 9001-78-9
• 효소 위원회 번호: 3.1.3.1 (BRENDA, IUBMB)
• EC Number: 232-631-4
• MDL number: MFCD00131849
• UNSPSC 코드: 12352204
• eCl@ss: 42010105
• NACRES: NA.54

속성

• 생물학적 소스: bovine (calf) intestinal mucosa
• 형태: solution (clear, colorless)
• 특이 활성도: ~2500 U/mg protein (~10 mg/ml)
• 분자량: M_r ~140000
• 기술: enzyme immunoassay: suitable
• 불순물: 0.1 mM ZnCl₂; 1 mM MgCl₂; 3 M NaCl; 30 mM triethanolamine
• pH: ~7.6
• 저장 온도: 2-8°C

설명

애플리케이션

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

생화학적/생리학적 작용

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

단위 정의

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

재구성

The solution can be used without dialysis (for enzyme immuno-assay).

기타 정보

Sales restrictions may apply

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)